AB Sciex API 5000

Available from KRSS a used fully refurbished and serviced AB-SCIEX API 5000 mass spectrometer.

The API 5000 LC/MS/MS System is a sensitive triple quadrupole mass spectrometer for small molecule analysis.

  • Overview
  • Features of the Instrument
  • Why purchase used refurbished
    mass specs from KRSS
  • Find Out More


The API 5000 system is designed for quantitative analysis of small molecules.

This application involves measurement of specific molecular weight compounds, usually a drug or metabolite in urine or other body fluid, and their resulting fragment ions for determining the exact quantity of the compound in the sample of interest. Quantitation is performed using a standard curve of mass spectrometer signal intensity for various known concentrations of the compound. The signal in the test sample is compared to the standard curve to determine the concentration.

The typical scan type used for this application in the API 5000 system, as well as all triple quadrupole instruments, is MRM (Multiple Reaction Monitoring).

Features of the Instrument

The API 5000 LC/MS/MS system combines all of the features of the API 4000™ LC/MS/MS system with the additional benefits of improved sensitivity and enhanced signal-to-noise ratio. It allows comprehensive analysis of biopharmaceutical compounds and it has the specificity needed for new drug development. It has the following features:

  • A wide diameter intake orifice which improves sensitivity by flowing more ions into the vacuum chamber.
  • A QJet® ion guide that prefocuses the ions before they enter the vacuum chamber for higher sensitivity and signal-to-noise ratio.
  • Improved Q2 lenses reduce CAD gas leakage into the vacuum chamber and improve sensitivity and signal-to-noise ratio.
  • Higher quadrupole operating frequency, which improves ion transmission and increases sensitivity, especially for low ion masses.
  • Higher sensitivity in all RF/DC scan modes.
  • Scanning in a mass range of m/z 5 - 1250 in quadrupole mode.
  • Enhanced resolution at reduced scan speeds.
  • Detector type: Pulse Counting CEM
  • Ionization Sources: TurboIonSpray, APCI, DuoSpray, PhotoSpray
  • Scan types: Q1 MS, Q3 MS, Product Ion, Precursor Ion, Neutral Loss or Gain, MRM
  • Highest sensitivity for small molecule analysis
  • Lowest limits of detection for DMPK and ADMET studies
  • Robust, high-throughput platform for all stages of pharmaceutical development
  • Accuracy and precision for quantitative analysis
  • Eliminates Cross-Contamination

For pharmaceutical and pharmacokinetic samples, MS/MS has the sensitivity and specificity needed to analyse hundreds of samples per day without requiring extensive sample preparation.

Why purchase used refurbished
mass specs from KRSS

KRSS Ltd offer high quality pre-owned Mass Spec, LCMS, GCMS, HPLC and GC systems allowing affordable entry into a rapidly developing market.

All KRSS instruments are thoroughly inspected by manufacturer trained engineers (including all components and parts), cleaned and decontaminated, completely refurbished (defective parts and components are replaced with genuine parts), serviced and bench tested to ensure the equipment meets the manufacturers original specifications - this guarantees quality, performance and reliability at a fraction of the OEM costs.

With over 50 instruments in stock, covering all major manufacturers KRSS has systems for a variety of application requirements. All instruments are supplied with a comprehensive warranty and optional service contracts to ensure trouble free operation.


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