Thermo TSQ Vantage

Now available from KRSS, a used fully refurbished and serviced Thermo TSQ Vantage Triple Quad with 3 months warranty and installation.

  • Overview
  • Performance Specifications
  • Hardware Features
  • Product Specifications
  • System Specifications
  • Find Out More

Overview

The Thermo Scientific TSQ Vantage is the top-of-the-line model of the Thermo Scientific triple stage quadrupole (TSQ) mass spectrometer product line.
 
The TSQ Vantage™ incorporates the sensitive and rugged Thermo Scientific Ion Max source with the newly developed G2 ion optics. In conjunction with our precision hyperbolic quadrupoles, which provide the best transmission and peak shape, our detection technology and ion source results in the most sensitive triple quadrupole instrument available today.

State-of-the-art electronics, comprehensive diagnostics, and easy-to-use Thermo Scientific Xcalibur software give you total instrument control at your fingertips.

With these industry-leading features, the TSQ Vantage defines a new standard of excellence in bioanalytical, environmental, clinical research, and quantitative proteomic analysis.


  • Superior precision with Generation 2 (G2) ion optics
  • Easier to use with smarter software
  • Simultaneous quantitation and confirmation with QED-MS/MS
  • Up to 3000 SRMs definable per run
  • Scan speeds: up to 5000 u/s

Performance Specifications

Mass Range

  • m/z 10-1500 daltons (Da) (TSQ Vantage and TSQ Vantage AM)
  • m/z 10-3000 Da (TSQ Vantage EMR)

Resolution

  • 7500 (FWHM) at m/z 508 of polytyrosine
  • Resolution is continuously adjustable to better than 0.1 Da peak width (FWHM) across the entire mass range


Mass Stability

  • Mass assignment will be within ± 0.050 Da over a 24-hour period. The laboratory room temperature must be maintained between 15-27 °C (59-81°F). The optimum temperature of operation is between 18-21°C (65-70 °F). The room temperature may not change by more than 5 °C (9 °F) during this period.


Hardware Features

Ion Max™ API Source

  • Enhanced precision and ruggedness
  • Sweep gas reduces chemical noise
  • Optimal 60-degree spray angle
  • Interchangeable HESI-II and APCI ionization probes
  • Removable ion transfer tube provides vent-free maintenance
  • High temperature, self-cleaning APCI heater employing state-of-the-art ceramic heater technology
  • Dual Desolvation Zone Technology with the HESI-II
  • G2 ion optics for improved ion transmis- sion
  • X, Y, and Z probe positioning adjust- ments for all ionization probes
  • Automatic source recognition for ease of use and simplified data logging
  • Square profile quadrupole ion guide for the highest ion transmission

Product Specifications

Triple Stage Quadrupole

  • Dual Thermo Scientific HyperQuad preci- sion hyperbolic quadrupole mass analyzers for ultra performance
  • Large 6 mm field radius provides high transmission and superior peak shape
  • 90-degree square profile quadrupole rod assembly ion guide with noise-reducing geometry
  • Software control and automated optimiza- tion of collision energy and gas pressure
  • Fully automated system calibration, tuning, and compound optimization


Vacuum System

  • Four-stage differentially pumped vacuum manifold
  • Advanced triple inlet turbomolecular pump integrated with vacuum manifold
  • Dual rotary vacuum pump configuration


Detection System

  • Off-axis continuous dynode electron multiplier with extended dynamic range
  • ±15 kV post-acceleration conversion dynode
  • Digital electronic noise reduction
  • System integrated electron multiplier eliminates field emission and microphonic noise


Integrated Divert Valve

  • Fully automated data system control enables switching the solvent front, gradient end point, or any portion of the HPLC run to waste
  • Capable of automatic system optimization by loop injections
  • User-definable default state of the valve, either “to waste-load” or “to source-inject”


Integrated Syringe Pump

  • Automated infusion and loop injections under full data system control


Source Options

  • HESI-II source compatible with liquid flow rates of 1 μL/min to 2 mL/min, without splitting
  • APCI/APPI source compatible with liquid flow rates of 50 μL/min to 2 mL/min, without splitting
  • Metal needle configurations for low-flow or high-flow analysis
  • NanoSpray source supports both static and dynamic nanospray experiments, compatible with liquid flow rates of 50 nL/min* to 50 μL/min
  • FAIMS reduces noise and matrix interferences, resulting in improved assay robustness and increased assay sensitivity

*Lower limit depends on gauge of needle used.

Scan Functions

  • Highly sensitive full-scan MS in Q1 or Q3
  • Selected Ion Monitoring (SIM) in Q1 or Q3
  • Timed Selected Reaction Monitoring (SRM) for the most demanding bioanalyti- cal and proteomics assays. Up to 3000 SRMs definable.
  • Product Ion Scanning
  • Precursor Ion Scanning
  • Neutral Loss Scanning
  • H-SRM
  • Reverse Energy Ramp MS/MS spectra (RER) gives information-rich MS/MS spectra for solid compound identification


Advanced Experiments with Data- Dependent Acquisition Mode

  • Available from all scan types
  • Thermo Scientific Dynamic Exclusion allows acquisition of MSn spectra from lower intensity ion species
  • Polarity switching capabilities
  • AutoSIM
  • AutoLock feature for accurate mass systems
  • Quantitation-Enhanced Data-Dependent MS/MS (QED-MS/MS) for simultaneous compound confirmation and quantitation


System Control

  • Powerful embedded computer system with Motorola PowerPC® processor
  • Integrated Serial Peripheral Interconnect (SPI) bus for reliable electronic communi- cations between system modules
  •  Dedicated SHARC® digital signal proces- sor (DSP) for dedicated instrument control
  • Fast 100BASE-T Ethernet port for PC-to- instrument communications


Data Acquisition

  • Real-time, high-speed, digital signal processing with dedicated SHARC digital signal processor (DSP)
  • High-speed analog-to-digital converter (ADC) with 195 kHz sampling rate
  • Adjustable scan speeds to 5000 u/s
  • High-resolution centroiding

System Specifications

Sensitivity
Electrospray (HESI) at Unit Resolution A 5 μL injection of a 100 fg/μL (0.3250 fmol/μL) reserpine solution on a Hypersil
GOLD aQ™ 20 X 2.1 mm 1.9 μm particle
packed column at a flow rate of 500 μL/min 30:70:0.1 water/Acetonitrile/Formic acid will produce a minimum signal-to-noise ratio of 500:1 for the transition of the proto- nated molecule at m/z 609.3 to the frag- ment ion at m/z 195.1 when operated in selected reaction monitoring mode (SRM) with Q1 and Q3 resolution set to 0.7 Da FWHM.

Heated Electrospray (HESI) at High Resolution
A 5 μL injection of a 100 fg/μL (0.3250 fmol/μL) reserpine solution on a Hypersil GOLD aQ 20 X 2.1 mm 1.9 μm particle packed column at a flow rate of 500 μL/min 30:70:0.1 water/Acetonitrile/Formic acid will produce a minimum signal-to-noise ratio of 500:1 for the transition of the proto- nated molecule at m/z 609.3 to the frag- ment ion at m/z 195.1 when operated in selected reaction monitoring mode (SRM) with Q1 resolution set to 0.2 Da FWHM and Q3 resolution set to 0.7 Da FWHM.

Atmospheric Pressure Chemical Ionization (APCI) and Atmospheric Pressure Photoionization (APPI) at Unit Resolution
A 5 μL injection of a 100 fg/μL (0.3250 fmol/μL) reserpine solution on a Hypersil GOLD aQ 20 X 2.1 mm 1.9 μm particle packed column at a flow rate of 500 μL/min 30:70:0.1 water/Acetonitrile/Formic acid will produce a minimum signal-to-noise ratio of 500:1 for the transition of the proto- nated molecule at m/z 609.3 to the frag- ment ion at m/z 195.1 when operated in selected reaction monitoring mode (SRM) with Q1 and Q3 resolution set to 0.7 Da FWHM.

APCI and APPI at High Resolution
A 5 μL injection of a 100 fg/μL (0.3250 fmol/μL) reserpine solution on a Hypersil GOLD aQ 20 X 2.1 mm 1.9 μm particle packed column at a flow rate of 500 μL/min 30:70:0.1 water/Acetonitrile/Formic acid will produce a minimum signal-to-noise ratio of 500:1 for the transition of the proto- nated molecule at m/z 609.3 to the frag- ment ion at m/z 195.1 when operated in selected reaction monitoring mode (SRM) with Q1 resolution set to 0.2 Da FWHM and Q3 resolution set to 0.7 Da FWHM.

Demo

 
KRSS Left Strip