Thermo Orbitrap Fusion Lumos

Now available from KRSS, a used fully refurbished and serviced Thermo Orbitrap Fusion Lumos Tribrid Mass Spectrometer.

  • Overview
  • Features
  • Specifications
  • Find Out More

Overview

Excel at the most challenging of applications, including low level PTM analysis, multiplexed relative quantitation using isobaric tags, intact protein characterization, as well as MSn analysis of small molecules with the new Thermo Scientific Orbitrap Fusion Lumos Tribrid Mass Spectrometer. This system incorporates the brightest ion source, a segmented quadrupole mass filter with improved selectivity and ion transmission, Advanced Vacuum Technology for improved ion transmission to the Thermo Scientific Orbitrap mass analyzer, and ETD HD, a higher-capacity ETD fragmentation.

The latest innovations include the Advanced Peak Determination (APD) algorithm for improved data-dependent experiments and two new hardware options: UVPD, a new fragmentation technique achieved with a 213 nm UV laser and 1M, which provides 1,000,000 FWHM ultra-high resolution for improved structural elucidation and quantitation of isobaric compounds.

latest innovations in Orbitrap Fusion Lumos Technology:

  • EASY-IC option for improved mass-to-charge ratio (m/z) assignment
  • EASY-ETD option to efficiently fragment peptides and proteins
  • UVPD option to fragment the most challenging compounds
  • 1,000,000 FWHM ultra-high resolution for in-depth characterization

Features

The newest Tribrid instrument takes advantage of the combination of a brighter ion source, segmented quadrupole, improved ion transmission and improved ETD performance. It is based on the Orbitrap Fusion platform and contains new hardware and software features specifically developed to improve performance in the most challenging applications in biological research areas, including proteomics, metabolomics and biopharma. They include, but are not limited to:


  • A brighter ion source provided by a High Capacity Transfer Tube and an Electrodynamic Ion Funnel for increased sensitivity
  • Advanced Active Ion Beam Guide for improved robustness
  • Segmented quadrupole powered by Advanced Quadrupole Technology for improved precursor ion selection and transmission
  • Advanced Vacuum Technology for improved vacuum in the C-trap and Orbitrap regions resulting in improved ion transfer to the Orbitrap mass analyzer
  • ETD HD (High Definition ETD) incorporating a higher capacity ion-ion reaction for improved dynamic range of ETD spectra

Specifications

Mass Range

m/z 50 - 2,000
m/z 200 - 4,000
up to m/z 6,000 without precursor ion
selection (OTMS only)

Resolution OTMS
15,000 - 500,000 (FWHM) at m/z 200

Scan Rate*
OTMSn up to 20 Hz
ITMSn up to 20 Hz

Mass Accuracy OTMS*
< 3 ppm RMS using external calibration
< 1 ppm RMS using internal calibration

MS/MS Electrospray Ionization (ESI) Ion
Trap sensitivity:

2 μL of a 50 fg/μL solution of reserpine (100 femtograms total) injected at a flow of 500 μL/min will produce a minimum signal-to-noise ratio of 200:1 for the transition of the isolated protonated molecular ions at m/z 609 to the largest two product ions, m/z 397 and m/z 448, when the mass spectrometer is operated at unit resolution in the full scan MS/MS mode, m/z 165 - 615. The test requires HESI III probe.

Dynamic Range
> 5,000 within a single OTMS spectrum

MS Scan Power
MSn, for n = 1 through 10

Synchronous Precursor Isolation
Up to 20 precursors per MSn scan

Multiplexing Using the Ion Routing Multipole
Up to 10 precursors per scan using the quadrupole mass filter

Polarity Switching
One full cycle in < 1.1 sec (one full scan positive mode and one full scan negative mode at resolution setting of 30,000)

ETD Efficiency
ETD fragmentation efficiency of >15%, (infusion of 1 pmol/μL angiotensin at 3 μL/min)

Analog Inputs
Channel 1 analog input (0 - 10 V),
Channel 2 analog (0 - 2 V)

*Under defined conditions

Demo

 
KRSS Left Strip