Thermo LTQ Velos Obritrap Velos

Now available from KRSS, a used fully refurbished and serviced Thermo LTQ Velos/Orbitrap Velos with ETD, nano and standard source including chiller, vacuum pumps, PC, Waters Nano Acquity UPLC system, 3 months warranty, installation and training.

  • Overview
  • Technical Data
  • ETD System
  • Why purchase used refurbished instruments from KRSS
  • Find Out More

Overview

LTQ Orbitrap Velos is a hybrid mass spectrometer incorporating the LTQ Velos™ dual cell linear trap and the Orbitrap™ analyzer.

The LTQ Orbitrap Velos consists of four main components, which are described in the following topics:
  • A dual cell linear ion trap (Thermo Scientific LTQ Velos) for sample ionization, selection, fragmentation, and AGC™.
  • An intermediate storage device (curved linear trap) that is required for short pulse injection.
  • An Orbitrap analyzer for Fourier transformation based analysis.
  • A collision cell for performing higher energy CID experiments.

The LTQ Orbitrap Velos ETD has an additional reagent ion source for enabling post-translational modification analyses of peptides by Electron Transfer Dissociation (ETD).

Technical Data

Resolution
60 000 (FWHM) @ m/z 400 with a scan repetition rate of 1 second
Minimum resolution 7500, maximum resolution 100 000 @ m/z 400

Cycle Time
1 scan at 60 000 resolution @ m/z 400 per second

Mass Range
m/z 50 - 2000; m/z 200 - 4000

Mass Accuracy
<3 ppm RMS for 2 h period with external calibration using defined conditions,
<1 ppm RMS with internal calibration

Dynamic Range
>10 000 between mass spectra, >5000 between highest and lowest detectable ion signal in one spectrum

MS/MS
MS/MS and MSnscan functions

ETD System

In the LTQ Orbitrap Velos ETD, an ETD Module is physically coupled to the back of the LTQ Orbitrap Velos. A quadrupole mass filter replaces the octapole of the LTQ Orbitrap Velos. The linear trap provides the voltages for the quadrupole mass filter. A tube, which contains the transfer multipole, connects the HCD housing to the ETD Module. The ETD Ion Optic Supply board is mounted on top of the data acquisition unit on the right side of the instrument.

Protein or peptide analyte ions may also be fragmented in the linear trap by negatively charged reagent ions (fluoranthene radical anions) from the reagent ion source (ETD Module). These negatively charged ions deliver electrons to protein or peptide analyte ions and cause them to fragment at their peptide bonds to produce c and z type fragments (versus the y and b type fragments produced by CID). The resulting analyte fragment ions provide another way of analyzing these molecules as compared to CID and PQD. Electron Transfer Dissociation (ETD) improves the identification of important post-translational modification (PTM) for characterization.

Why purchase used refurbished instruments from KRSS

KRSS Ltd offer high quality pre-owned Mass Spec, LCMS, GCMS, HPLC and GC systems allowing affordable entry into a rapidly developing market.

All KRSS instruments are thoroughly inspected by manufacturer trained engineers (including all components and parts), cleaned and decontaminated, completely refurbished (defective parts and components are replaced with genuine parts), serviced and bench tested to ensure the equipment meets the manufacturers original specifications - this guarantees quality, performance and reliability at a fraction of the OEM costs.

With over 50 instruments in stock, covering all major manufacturers KRSS has systems for a variety of application requirements. All instruments are supplied with a comprehensive warranty and optional service contracts to ensure trouble free operation.

Demo

 
KRSS Left Strip