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Bruker HCT-Ultra ETD II

Now available from KRSS is a used, fully refurbished and serviced Bruker HCT Ultra ETD II and Dionex Ultimate 3000 with UV and N2 generator.

The LC system is composed by the following units; Solvent Rack / Degasser (SRD3600, Pumps, DGP-3600M, VWD3400 Detector, Flow manager control (FLM3100 nano 2X2P-10P), Autosampler (WPS-3000TPL nano, cap autosampler).

For today’s challenges in proteomics and metabolomics work, the HCT series is best prepared to partner the researcher in the fields of: Protein ID and Detailed Characterization Metabolite ID and Profiling General Chemistry LC/MSn.

Full scan MS sensitivity is a must for all data dependant experiments, it enables intelligent acquisition of MS/MS data especially on low abundance precursor ions.

Together with the dramatic increase in MS/MS data sets per unit time, HCT series instruments assure maximum information from your samples, optimizing protein identification and database scoring.

HCT series spherical ion traps provide great MS and MS/MS spectra quality - in terms of mass resolution, sensitivity and accuracy throughout a wide mass range at ultra fast HPLC speed.

ETD II is an electron transfer dissociation module for Bruker’s HCTUltra high capacity ion trap.

It is based on a negative chemical ionisation source with increased ETD/PTR reactant ion generation, resulting in rapid characterisation of PTMs such as phosphorylations or glycosylations at sub-fmol sensitivity.

ETD is a very powerful peptide and protein fragmentation technique that preserves functionally important post-translational modifications (PTMs), such as phosphorylations or glycosylations. With ETD in a spherical high-capacity ion trap, the sequencing and identification of proteins, as well as the identification and localization of PTMs are facilitated with greatly improved scores and confidence.

HCTUltra’s inherent m/z range of greater than 3,000 and its mass resolution that can resolve three+ and four+ charge states of peptides on the fly at full analytical speed means ETD/PTR on the HCTUltra can be used for up to 12 kDa proteins and beyond.

  • Overview
  • Why choose Bruker HCT Ultra ETD II
  • Why purchase used refurbished
    mass specs from KRSS
  • Find Out More

Overview

Ultra Performance High Capacity Trap

Set a new standard for high-information content LC-MSn!
For today’s challenges in proteomics and metabolomics work, the HCTultra is best prepared to partner the researcher in the fields of:

  • protein identification
  • protein characterization
  • de novo peptide sequencing
  • differential proteomics
  • metabolite profiling

The new cutting-edge HCTultra™ adds the best ever speed and sensitivity available anywhere to the trusted portfolio of LC-ESI-MSn ion traps from Bruker Daltonics.

Why choose Bruker HCT Ultra ETD II

Sensitivity & Speed
The HCTultra is one of the most sensitive ion trap on the market for MS and MS/MS scans. Full scan MS sensitivity is a must for all data dependant experiments, it enables intelligent acquisition of MS/MS data especially on low abundant precursor ions. Together with the dramatic increase in MS/MS peptide data per time unit the HCTultra assures maximum information from your samples, leading to a remarkable increase in peptide/protein identifications and database scoring.

Intelligence
Simultaneously the quality of MS/MS data reaches new heights due to new designs of smart precursor selection tools. The new PhosphoScan™ can easily detect phosphopeptides in a single LC-MS/MS run. Besides the well-established Smart ICC technology, PeptideScan™ allows on-the-fly selection of 2+/3+ charged peptide ions. For small molecule applications, SmartFrag™ and fast +/- switching provides highly reproducible MSn spectra for Librarysearch.

Precision
With the best mass accuracy available among ion traps, the HCTultra is an excellent instrument for de novo sequencing applications.

Equipped for transforming speed into information
Millions of MS/MS spectra, acquired with ease by HCTultra, are handled conveniently by ProteinScape™ the most sophisticated bioinformatics tools available. Furthermore, true non-redundant protein lists and reports are generated and fully automated screening for a large set of modifications may be initiated. Even automated phosphopeptide detection is possible with the new HCTultra.

Bruker Daltonics again redefines the leadership in LC based MSn instruments with the new HCTultra. Unrivalled speed, sensitivity and mass accuracy are complemented with smart, sophisticated software tools to get the most out of every important sample.

Scan modes

  • Full scan MS and MS/MS in all scan modes for sensitive and fast analysis of unknown compounds.
  • PhosphoScan™: Neutral loss dependant AutoMS3 scans e.g. for determination of protein modifications.
  • on-the-fly selection of 2+/3+ charged peptide ions with PeptideScan™
  • Multiple reaction monitoring (MRM) with MS/MS and MS3.
  • Manual MSn up to MS11 in all scan modes.
  • AutoMSn tree experiments with up to 30 MS precursor ions and 5 fragment ions per stage.
  • Triple Play experiments without time consuming "charge-state determination-scan"

Data-dependent experiments

All types of data-dependent experiments, including preferred mass list for automated feedback experiments from MetaboliteTools, PROTEINEER and PROTEINEER-LC.

Why purchase used refurbished
mass specs from KRSS

KRSS Ltd offer high quality pre-owned Mass Spec, LCMS, GCMS, HPLC and GC systems allowing affordable entry into a rapidly developing market.

All KRSS instruments are thoroughly inspected by manufacturer trained engineers (including all components and parts), cleaned and decontaminated, completely refurbished (defective parts and components are replaced with genuine parts), serviced and bench tested to ensure the equipment meets the manufacturers original specifications - this guarantees quality, performance and reliability at a fraction of the OEM costs.

With over 50 instruments in stock, covering all major manufacturers KRSS has systems for a variety of application requirements. All instruments are supplied with a comprehensive warranty and optional service contracts to ensure trouble free operation.

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