The Clarity PDA Extension is a tool that is used for processing data that has been acquired from selected photo diode array detectors. Spectral data, together with chromatograms, add a third dimension to analytical data analysis.
The Clarity PDA Extension expands the capability of Clarity Chromatography software by providing interactive spectral analysis, peak purity analysis and compound identification that is based on a spectral library search. PDA data can be displayed in a set of optional views including 3D rendering.
The PDA Extension is an optional Extension for the Clarity Chromatography Station (starting from version 2.4) and Clarity Offline Chromatography Station. The Clarity Chromatography Station is designed to acquire and evaluate data from up to four chromatographs at a time (multi-detector measurement support). Any Instrument within the Clarity station can use the PDA Extension.
All data is saved in a single file; a chromatogram at any wavelength or even only a spectrum can simply be recalled for review after an analysis. UV-VIS spectra, acquired using a PDA detector, may be interactively selected from a chromatogram signal for visual inspection and comparison. The spectra may also be used for peak purity determinations, wavelength optimization and component identification through spectral libraries.
A control modules for selected detectors (see list of control modules) are available. Additional control modules for other PDA detectors can be developed upon request.
The Clarity PDA Extension expands the capability of Clarity Chromatography software providing interactive spectral analysis, peak purity analysis and compound identification that is based on spectral library search.
The PDA view is customizable; up to four views can be displayed at one time (Any combination from the following views may be selected: Isoplot, Chromatogram, Spectra, 3D Display, Peak Purity, Peak Purity Spectra, Library Spectra Search and Spectral Library). The user can easily extract chromatographic signals from PDA data to determine the optimal detection wavelength for each peak.
The Clarity PDA method includes an option for Spectral Library Search and Peak Purity analysis.
The Clarity software compares the peak spectra with the spectra of an unlimited number of spectral libraries. Spectra stored in a Spectral Library include retention times and analysis parameters (optional). The Spectral Library Search can perform automatic identification of integrated/calibrated components (peaks). The library search may be constrained by the RT Window and by Wavelength Range. Either the Least Square, the Weighted Least Square or the Correlation Method is used for calculating library search matches. A Background Correction option is also available.
This analysis helps to discover hidden impurities. Peak Purity test is applied to all integrated/calibrated peaks in the active signal. Purity is calculated from 5 or all spectra within the peak. The similarity curve is displayed in the PDA window. Peak Purity analysis can be optimized by setting custom preferences relating to the purity threshold, wavelength restriction and absorbance threshold.
Spectral data can be imported/exported in ASCII text formats to or from the Clarity software.
Users can easily include PDA options such as data 3D Display, Isoplot, Spectra, Peak Purity and Library Search results in reports using the intuitive Report Setup Dialog.
Control modules for Agilent 1100 DAD and FLD, Duratec DDT3200 and Knauer PDA are available. Additional control modules for other PDA detectors can be developed upon request.